Harris S D, Anobile C J, McLoughlin J D, Mitchell R, Lambert A, Robertson W R
Department of Medicine, University of Manchester, Hope Hospital, Salford, UK.
Mol Hum Reprod. 1996 Oct;2(10):807-11. doi: 10.1093/molehr/2.10.807.
Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were measured by radioimmunoassay and a two-site immunoradiometric assay and compared with those in two urinary preparations (Metrodin and Metrodin-HP) used in assisted reproduction programmes and a urinary FSH international standard 70/45 (uFSH IS 70/45). Similar data were obtained with both assays; rhFSH had 6% complex internal carbohydrate structures compared with 22-27% for Metrodin, Metrodin-HP and uFSH. The proportion of simple carbohydrate structures was also different, with rhFSH having 18.5 compared with 4.5-9.3% for Metrodin, Metrodin-HP and uFSH. A linear relationship was observed between the percentage glycoforms with an isoelectric point (pl) < 4 and the log percentage simple forms (logarithmic regression; r = 0.93) indicating a direct relationship between carbohydrate complexity and charge heterogeneity. In summary, rhFSH contains fewer complex forms and an increased proportion of simple carbohydrate structures in comparison with Metrodin, Metrodin-HP and IS 70/45.
利用伴刀豆球蛋白A凝集素亲和色谱法对重组人促卵泡激素（rhFSH）（Org 32489，果纳芬）的糖型进行表征，以揭示制剂内部碳水化合物复杂性（碳水化合物侧链分支程度）的相关信息。通过放射免疫测定法和双位点免疫放射测定法对rhFSH糖型进行测定，并与辅助生殖计划中使用的两种尿促卵泡素制剂（美诺孕和高纯度美诺孕）以及尿促卵泡素国际标准品70/45（uFSH IS 70/45）中的糖型进行比较。两种测定方法得到了相似的数据；rhFSH具有6%的复杂内部碳水化合物结构，而美诺孕、高纯度美诺孕和uFSH的这一比例为22 - 27%。简单碳水化合物结构的比例也有所不同，rhFSH为18.5%，而美诺孕、高纯度美诺孕和uFSH为4.5 - 9.3%。观察到等电点（pI）< 4的糖型百分比与简单糖型对数百分比之间存在线性关系（对数回归；r = 0.93），表明碳水化合物复杂性与电荷异质性之间存在直接关系。总之，与美诺孕、高纯度美诺孕和IS 70/45相比，rhFSH含有较少的复杂糖型和比例增加的简单碳水化合物结构。
