Carani C, Gromoll J, Brinkworth M H, Simoni M, Weinbauer G F, Nieschlag E
Chair of Endocrinology, University of Modena, Italy.
Mol Hum Reprod. 1997 Jun;3(6):479-83. doi: 10.1093/molehr/3.6.479.
A gene on the human Y chromosome, specifically deleted in azoospermic patients (DAZ: deleted in azoospermia), and a DAZ homologue (DAZH) on human chromosome 3, have been recently described. In the present work we report the isolation and characterization of the corresponding DAZH gene of the cynomolgus monkey (Macaca fascicularis), which we have named cynDAZLA (cynomolgus DAZ-like autosomal). Reverse transcription-polymerase chain reaction was used to amplify the monkey DAZ homologue, and sequence analysis revealed an open reading frame of 888 bp encoding 295 amino acids. Northern blot hybridization of different tissues to a probe derived from the cynDAZLA cDNA detected a transcript of 3.5 kb that, in the male, was expressed only in the testis. Comparison of the cynDAZLA sequence to autosomal DAZ homologues from human, mouse and Drosophila showed two RNA-recognition motifs (RRM) and the presence of only one DAZ consensus repeat compared with the seven repeats found in the human DAZ gene on the Y chromosome. The homology of the cynDAZLA cDNA compared with the human DAZH and mouse dazla cDNAs is 97.97 and 87.46% respectively. The identification of the monkey cynDAZLA enables further studies regarding the putative functions of DAZH, such as onset of expression and hormonal dependence of this gene.
人类Y染色体上一个在无精子症患者中特异性缺失的基因（DAZ：无精子症缺失基因），以及人类3号染色体上的一个DAZ同源物（DAZH），最近已被报道。在本研究中，我们报告了食蟹猴（猕猴）相应DAZH基因的分离和特性分析，我们将其命名为cynomolgus DAZ-like autosomal（cynomolgus DAZ样常染色体基因，简称cynDAZLA）。采用逆转录-聚合酶链反应扩增猴DAZ同源物，序列分析显示一个888 bp的开放阅读框，编码295个氨基酸。用源自cynDAZLA cDNA的探针与不同组织进行Northern印迹杂交，检测到一个3.5 kb的转录本，在雄性中仅在睾丸中表达。将cynDAZLA序列与人、小鼠和果蝇的常染色体DAZ同源物进行比较，发现有两个RNA识别基序（RRM），并且与Y染色体上人类DAZ基因中发现的七个重复序列相比，仅存在一个DAZ共有重复序列。cynDAZLA cDNA与人DAZH和小鼠dazla cDNA的同源性分别为97.97%和87.46%。食蟹猴cynDAZLA的鉴定有助于进一步研究DAZH的推定功能，如该基因的表达起始和激素依赖性。
