Witt B R, Pollard J W
Department of Obstetrics and Gynecology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Fertil Steril. 1997 Aug;68(2):259-64. doi: 10.1016/s0015-0282(97)81512-9.
To evaluate colony stimulating factor-1 (CSF-1) concentrations in serum and follicular fluid (FF) at the time of oocyte retrieval and to test for presence of messenger RNA (mRNA) for CSF-1 and its receptor, c-fms, in FF cells.
Collection of serum and FF at the time of oocyte retrieval.
A university IVF program.
PATIENT(S): Forty-five women undergoing oocyte retrieval for IVF.
INTERVENTION(S): Serum and FF were obtained from 24 women, and FF only was obtained from 21 women.
MAIN OUTCOME MEASURE(S): Colony-stimulating factor-1 concentrations were determined by RIA, and the presence of mRNA for CSF-1 and c-fms was determined by reverse transcriptase-polymerase chain reaction.
RESULT(S): Mean FF concentrations of CSF-1 were significantly higher than mean serum levels (10.0 +/- 1.3 and 3.6 +/- 0.3 (+/-SE) ng/mL, respectively). Colony-stimulating factor-1 and c-fms message were detected in FF cells, and alternatively spliced forms of CSF-1 message were present.
CONCLUSION(S): The presence of CSF-1, a primary regulator of tissue macrophages, in FF, and the presence of mRNA for CSF-1 and its receptor c-fms in FF-derived cells, suggest a role for this growth factor in ovarian function.
