The effect of high and low Dk/L soft contact lenses on the glycocalyx layer of the corneal epithelium and on the membrane associated receptors for lectins.

PURPOSE

Bacterial adherence or binding to the target cell is a prerequisite for the initial stage of most infections and seems to be mediated by lectin-like ligands on the bacterial surface and specific receptors on the target cell membrane. The purpose of this study was to establish whether contact lens wear under closed eye conditions changes the glycocalyx layer, whether it exposes more lectin receptors than eye closure without a contact lens, and whether wear of low oxygen transmissibility (Dk/L) contact lenses exposes more receptors than high Dk/L contact lenses.

METHODS

The eyes of six rabbits under general anesthesia were fit with either a high Dk/L soft contact lens (40 x 10(-9), boundary corrected) or a low Dk/L soft contact lens (2 x 10[-9]) or were left without a lens as controls. All eyes were kept closed by suturing for 24 hours. After removal of the contact lenses, all corneas were excised, put in glutaraldehydeforfixation, rinsed, incubated with plant-derived lectins (wheat-germ agglutinin [WGA]) conjugated with gold particles, and prepared for electron microscopy. Membrane associated gold particles were counted and the results were processed statistically.

RESULTS

After 24 hours of lens wear under closed eye conditions, the glycocalyx layer showed physical changes in the form of thinning or compression and signs of biochemical changes reflected as an increase in number of WGA receptors. The average number of membrane associated gold particles per 750 micro length of corneal epithelium in control corneas was 1,287.5 +/- 92.5. Particles were significantly (P<0.001) more numerous after wear of high Dk/L contact lenses (3,230.0 +/- 294.5) and after wear of low Dk/L contact lenses (4,611.3 +/- 223.3). The figure after wear of low Dk/L contact lenses was significantly (P<0.01) higher than the figure after wear of high Dk/L contact lenses.

CONCLUSION

Our results indicate that lens wear under closed eye conditions seems to change the corneal glycocalyx layer physically as well as biochemically. Significantly larger numbers of WGA receptors were exposed after contact lens wear than without a contact lens. Significantly more receptors were exposed after wear of low Dk/L contact lenses than after wear of high Dk/L contact lenses. These changes may be of importance in relation to the risk of bacterial keratitis.