Ombrellaro M P, Stevens S L, Sciarrotta J, Schaeffer D, Freeman M B, Goldman M H
Department of Surgery, University of Tennessee Medical Center, Knoxville 37920, USA.
Am J Surg. 1997 Jul;174(1):29-32. doi: 10.1016/S0002-9610(97)00049-4.
To determine if a complete intra-arterial environment affects endothelialization rate and basement membrane organization in polytetrafluoroethylene (PTFE) grafts.
Thirty dogs underwent either infrarenal abdominal aorta PTFE interposition (12) or intraluminal stented (18) grafting. Grafts were explanted at 4 and 8 weeks and rate of endothelial ingrowth calculated. Endothelial cells were identified and basement membrane organization assessed using antibodies against endothelial cell-specific surface antigen CD31, type IV collagen, and laminin.
Endothelialization rates, expressed as percent graft surface area coverage per week, were 3.7% +/- 0.62% (4-week control), 12.9% +/- 0.58% (4-week stented), 4.2% +/- 0.62% (8-week control), and 10.0% +/- 0.54% per week (8-week stented grafts). Endothelial repaving rates were constant for control and increased in all stented grafts (P <0.01). At 4 weeks, laminin was identified in all control (6 of 6) and no stented grafts. Staining was confined to the 20% of the hyperplastic intimal area immediately below the endothelium. At 4 weeks, type IV collagen was present throughout the entire hyperplastic intima in control specimens but was confined to a discrete subendothelial zone in stented grafts. By 8 weeks, type IV collagen became concentrated in the luminal one third of the intima in control grafts.
Intra-arterial graft location is associated with early formation of an organized basement membrane and accelerated endothelialization in PTFE grafts.
确定完整的动脉内环境是否会影响聚四氟乙烯（PTFE）移植物的内皮化速率和基底膜组织。
30只犬接受了肾下腹主动脉PTFE置入术（12只）或腔内带支架移植术（18只）。在4周和8周时取出移植物，计算内皮细胞向内生长的速率。使用针对内皮细胞特异性表面抗原CD31、IV型胶原和层粘连蛋白的抗体来识别内皮细胞并评估基底膜组织。
以内皮化速率表示为每周移植物表面积覆盖率，分别为3.7%±0.62%（4周对照组）、12.9%±0.58%（4周带支架组）、4.2%±0.62%（8周对照组）和每周10.0%±0.54%（8周带支架移植物）。对照组的内皮重新铺覆速率恒定，所有带支架的移植物中该速率均增加（P<0.01）。在4周时，在所有对照移植物（6/6）中均检测到层粘连蛋白，而带支架的移植物中未检测到。染色局限于内皮下方增生内膜区域的20%。在4周时，IV型胶原在对照标本的整个增生内膜中均有分布，但在带支架的移植物中局限于不连续的内皮下区域。到8周时，IV型胶原在对照移植物内膜的腔内三分之一处集中。
动脉内移植物位置与PTFE移植物中有序基底膜的早期形成和内皮化加速有关。
