Chen Y P, Woods G M, Dandie G W, Muller H K
Division of Pathology, University of Tasmania, Hobart, Australia.
Immunol Cell Biol. 1997 Jun;75(3):238-44. doi: 10.1038/icb.1997.37.
The ability to produce antigen-specific down-regulation of an established immune response was investigated in 2,4,6-trinitrochlorobenzene (TNCB)-immune mice by delivery of antigen through chemical carcinogen- or ultraviolet B (UVB)-treated skin. When TNCB-immune mice were treated on the dorsal trunk skin with 7,12-dimethylbenz(a)anthracene (DMBA) followed by TNCB there was an antigen-specific reduction in both contact sensitivity and antibody production. Further, immune mice that received spleen cells from naive syngeneic donors treated with DMBA followed by TNCB also exhibited a reduction in both contact sensitivity and antibody production. In contrast, mice treated with UVB irradiation followed by TNCB had a reduction in contact sensitivity but not antibody production. These results provide evidence that an ongoing immune response can be manipulated by immunization through a modified skin immune system. This may provide a beneficial approach for the treatment of autoimmune disease.
通过经化学致癌物或紫外线B（UVB）处理的皮肤递送抗原，在2,4,6-三硝基氯苯（TNCB）免疫的小鼠中研究了产生抗原特异性下调已建立免疫反应的能力。当用7,12-二甲基苯并(a)蒽（DMBA）处理TNCB免疫小鼠的背部躯干皮肤，随后再给予TNCB时，接触敏感性和抗体产生均出现抗原特异性降低。此外，接受经DMBA处理后再给予TNCB的同基因未免疫供体脾细胞的免疫小鼠，其接触敏感性和抗体产生也均降低。相比之下，经UVB照射后再给予TNCB的小鼠接触敏感性降低，但抗体产生未受影响。这些结果证明，可通过经修饰的皮肤免疫系统进行免疫接种来操纵正在进行的免疫反应。这可能为自身免疫性疾病的治疗提供一种有益的方法。
