Leont'ev V K, Petrovich Iu A, Kruglova L N
Stomatologiia (Mosk). 1997;76(3):5-11.
Effects of the principal mineral and mineralizing components of the saliva, nutrient organic acids (carbohydrate and some others substances' metabolites) on the activity of microflora of soft dental deposit were studied in vitro. The inhibitory effect was assessed visually from the appearance or absence of deposit coating, pH measurement, and by photo- and nephelometry. Concentrations of lactic acid and sucrose were measured. The inactivating effects of the reagents were assessed by double control: qualitative, by comparing the specimen with the optic density of the extract from aqueous suspension of the deposit, and time control, by comparing the specimen with the optic density of extracts from deposit suspensions directly after mixing with the medium. Acid medium was found to block the formation of soft dental deposit and inhibit the production of lactic acid by bacterial deposit. Hence, weakly acid gargle is recommended as an effective and physiological measure preventing cariogenic situation resultant from the intake of easily fermenting carbohydrates.
在体外研究了唾液的主要矿物质和矿化成分、营养有机酸（碳水化合物及其他一些物质的代谢产物）对软牙菌斑微生物活性的影响。通过观察菌斑覆盖物的有无、测量pH值以及利用比浊法和分光光度法来评估抑制效果。测定了乳酸和蔗糖的浓度。通过双重对照评估试剂的灭活效果：定性对照，即将标本与菌斑水悬液提取物的光密度进行比较；时间对照，即将标本与刚与培养基混合后的菌斑悬液提取物的光密度进行比较。发现酸性介质可阻止软牙菌斑的形成，并抑制细菌菌斑产生乳酸。因此，建议使用弱酸漱口水作为一种有效且符合生理的措施，以预防因摄入易发酵碳水化合物而导致的致龋情况。
