Fromigué O, Marie P J, Lomri A
INSERM Unit 349, Cell and Molecular Biology of Bone Cartilage, Lariboisière Hospital, Paris, 75475, France.
Cytokine. 1997 Aug;9(8):613-23. doi: 10.1006/cyto.1997.0209.
We have studied the effects of rhTGF-beta2, dexamethanosome (dex) and 1,25(OH)2D3 on human bone marrow stromal (HBMS) cells in long-term culture. A fraction on HBMS expressed type I collagen (Col I) and osteopontin, and transient treatment (48 h) with dex increased the number of alkaline phosphatase (ALP) positive cells. Treatment with rhTGF-beta2 inhibited DNA synthesis and attenuated the stimulatory effect of dex on cell growth at 3-4 weeks of culture. 1,25(OH)2D3 inhibited DNA synthesis at 1-4 weeks, and dex partially blocked the inhibitory effect of 1,25(OH)2D3 on cell growth. rhTGF-beta2 and dex increased Col I synthesis at 1 week of culture. Both dex and 1,25(OH)2D3 increased ALP activity and mRNA levels independently, and when combined they had an additive or synergistic effect, whereas rhTGF-beta2 antagonized the stimulatory effect of dex on ALP activity. In addition, dex attenuated the increased osteocalcin expression induced by 1,25(OH)2D3. These results show that rhTGF-beta2, dex and 1,25(OH)2D3 have distinct effects and modulate the action of each other on human marrow stromal cell proliferation and differentiation at different time points during the culture.
我们研究了重组人转化生长因子β2（rhTGF-β2）、地塞米松（dex）和1,25-二羟维生素D3 [1,25(OH)2D3] 对长期培养的人骨髓基质（HBMS）细胞的影响。一部分HBMS细胞表达I型胶原蛋白（Col I）和骨桥蛋白，用dex短暂处理（48小时）可增加碱性磷酸酶（ALP）阳性细胞的数量。在培养3 - 4周时，用rhTGF-β2处理可抑制DNA合成，并减弱dex对细胞生长的刺激作用。1,25(OH)2D3在培养1 - 4周时抑制DNA合成，dex部分阻断1,25(OH)2D3对细胞生长的抑制作用。在培养1周时，rhTGF-β2和dex可增加Col I的合成。dex和1,25(OH)2D3均可独立增加ALP活性和mRNA水平，二者联合使用时具有相加或协同作用，而rhTGF-β2可拮抗dex对ALP活性的刺激作用。此外，dex可减弱1,25(OH)2D3诱导的骨钙素表达增加。这些结果表明，rhTGF-β2、dex和1,25(OH)2D3具有不同的作用，并在培养过程中的不同时间点相互调节对人骨髓基质细胞增殖和分化的作用。
