Corbett S J, Tomlinson I M, Sonnhammer E L, Buck D, Winter G
MRC Centre For Protein Engineering, Hills Road, Cambridge, CB2 2QH, U.K.
J Mol Biol. 1997 Jul 25;270(4):587-97. doi: 10.1006/jmbi.1997.1141.
We have determined the complete nucleotide sequence of the human immunoglobulin D segment locus on chromosome 14q32.3 and identified a total of 27 D segments, of which nine are new. Comparison with a database of rearranged heavy chain sequences indicates that the human antibody repertoire is created by VDJ recombination involving 25 of these 27 D segments, extensive processing at the V-D and D-J junctions and use of multiple reading frames. We could find no evidence for the proposed use of DIR segments, inverted D segments, "minor" D segments or D-D recombination. Conventional VDJ recombination, which obeys the 12/23 rule, is therefore sufficient to explain the wealth of lengths and sequences for the third hypervariable loop of human heavy chains.
我们已经确定了位于14号染色体q32.3区域的人类免疫球蛋白D片段基因座的完整核苷酸序列，并总共鉴定出27个D片段，其中9个是新的。与重链重排序列数据库的比较表明，人类抗体库是由涉及这27个D片段中25个的VDJ重组、V-D和D-J连接处的广泛加工以及多个阅读框的使用所产生的。我们没有找到关于所提出的DIR片段、反向D片段、“次要”D片段或D-D重组使用的证据。因此，遵循12/23规则的传统VDJ重组足以解释人类重链第三个高变环的丰富长度和序列。
