Yoo M
Department of Biology, Keimyung University, Taegu, Korea.
Biochem Biophys Res Commun. 1997 Jul 30;236(3):641-2. doi: 10.1006/bbrc.1997.7023.
Two kinds of PCR products were isolated from adult rat brain. The smaller product matched up to liver b5 mRNA completely. However, the larger one contained an additional 58 bp inserted between amino acids 96 and 97. This insertion was previously unknown and corresponded to in-frame codons for his-ser-ala-leu and the early stop. It is assumed that these two forms arise from a primary transcript by differential mRNA processing. This finding strongly suggests that there are two homologous b5s expressed in rat brain.
从成年大鼠大脑中分离出两种PCR产物。较小的产物与肝脏b5 mRNA完全匹配。然而，较大的产物在氨基酸96和97之间额外插入了58个碱基对。这种插入以前未知，对应于组氨酸-丝氨酸-丙氨酸-亮氨酸的读框内密码子和早期终止密码子。推测这两种形式是由初级转录本通过不同的mRNA加工产生的。这一发现强烈表明，大鼠大脑中表达了两种同源的b5。
