禽腺病毒在模型双层脂质膜中诱导离子通道。

Avian adenovirus induces ion channels in model bilayer lipid membranes.

作者信息

Rosenkranz A A, Antonenko Y N, Smirnova O A, Yurov G K, Naroditsky B S, Sobolev A S

机构信息

Department of Biophysics, Biological Faculty, Moscow State University, Russia.

出版信息

Biochem Biophys Res Commun. 1997 Jul 30;236(3):750-3. doi: 10.1006/bbrc.1997.7040.

DOI:10.1006/bbrc.1997.7040

PMID:9245727

Abstract

The action of duck egg drop syndrome 1976 (EDS-76) adenovirus on model bilayer lipid membranes (BLM) has been investigated on planar egg phosphatidylcholine membranes and small unilamellar vesicles. It was found that the adenovirus formed channels in planar BLM in a pH-dependent manner. The addition of EDS-76 to planar BLM at pH 5 induced voltage-independent channel activity of about 60 pS conductivity after a lag phase. At pH 3, EDS-76 induced irregular spikes of current across the planar BLM which disappeared after several minutes. The adenovirus also was able to induce pH-dependent leakage of calcein-loaded liposomes. EDS-76 did not induce channel activity in planar BLM or liposome leakage at neutral pH.

摘要

1976年鸭瘟病毒（EDS - 76）腺病毒对平面卵磷脂膜和小单层囊泡等模型双层脂质膜（BLM）的作用已被研究。发现腺病毒在平面BLM中以pH依赖的方式形成通道。在pH 5时向平面BLM中添加EDS - 76，经过延迟期后诱导出约60 pS电导率的电压非依赖性通道活性。在pH 3时，EDS - 76诱导平面BLM上出现不规则电流尖峰，几分钟后消失。腺病毒还能够诱导负载钙黄绿素的脂质体发生pH依赖的渗漏。在中性pH条件下，EDS - 76不会诱导平面BLM中的通道活性或脂质体渗漏。