[脂质体与平面脂质膜的融合]
[Liposome fusion with planar lipid membranes].
作者信息
Egorova E M, Chernomordik L V, Abidor I G, Chizmadzhev Iu A
出版信息
Biofizika. 1981 Jan-Feb;26(1):145-7.
PMID:7225443
Abstract
Potentiodynamic method was used for investigating bilayer lipid membrane (BLM)- liposomes (LS) interaction. BLM was formed from egg lecithin and its mixture with phosphatidylserine (PS); LS-from PS. It is shown that in the presence of calcium in the aqueous phase (1-10 mM) and charged phospholipids in both LS and BLM, fusion of LS with BLM is observed. The fusion event was registered by measuring the increase of negative charge density on BLM surface opposite to that facing the LS.
摘要
采用动电位法研究双层脂质膜（BLM）与脂质体（LS）的相互作用。BLM由卵磷脂及其与磷脂酰丝氨酸（PS）的混合物形成；LS由PS形成。结果表明，在水相中存在钙（1 - 10 mM）且LS和BLM中均存在带电磷脂的情况下，观察到LS与BLM的融合。通过测量BLM表面与面对LS一侧相反的一侧负电荷密度的增加来记录融合事件。
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