Cohen D, Michel J, Ferne M, Bergner-Rabinowitz S, Ginsburg I
Inflammation. 1979 Sep;3(4):395-403. doi: 10.1007/BF00913497.
Leukocyte extracts, trypsin, and lysozyme are all capable of releasing the bulk of the LPS from S. typhi, S. typhimurium, and E. coli. Bacteria which have been killed by heat, ultraviolet irradiation, or by a variety of metabolic inhibitors and antibiotics which affect protein, DNA, RNA, and cell wall synthesis no longer yield soluble LPS following treatment with the releasing agents. On the other hand, bacteria which are resistant to certain of the antibiotics yield nearly the full amount of soluble LPS following treatment, suggesting that certain heatlabile endogenous metabolic pathways collaborate with the releasing agents in the release of LPS from the bacteria. It is suggested that some of the beneficial effects of antibiotics on infections with gram-negative bacteria may be the prevention of massive release of endotoxin by leukocyte enzymes in inflammatory sites.
白细胞提取物、胰蛋白酶和溶菌酶都能够从伤寒沙门氏菌、鼠伤寒沙门氏菌和大肠杆菌中释放出大部分脂多糖。经加热、紫外线照射或多种影响蛋白质、DNA、RNA和细胞壁合成的代谢抑制剂及抗生素处理而死亡的细菌，在用释放剂处理后不再产生可溶性脂多糖。另一方面，对某些抗生素具有抗性的细菌在用释放剂处理后可产生几乎全部数量的可溶性脂多糖，这表明某些热不稳定的内源性代谢途径与释放剂协同作用，促使脂多糖从细菌中释放出来。有人提出，抗生素对革兰氏阴性菌感染的一些有益作用可能是防止炎症部位白细胞酶大量释放内毒素。
