Casu A, Saito A, Fleischer B, Fleischer S
Ital J Biochem. 1977 Jul-Aug;26(4):297-316.
Heavy beef heart mitochondria depleted of phospholipids by treatment with phospholipase C followed by removal of the by products by lipase treatment or sonication in pentane were analyzed by electron microscopy, chemical analysis and assays of enzymatic activities. The results indicate that diglycerides are present after phospholipase C treatment and are inhibitors of NADH-cytochrome c reductase. After removal of diglycerides with lipase treatment, a phospholipid requirement for NADH-cytochrome c reductase could be demonstrated.
用磷脂酶C处理使重牛心线粒体的磷脂耗尽，然后通过脂肪酶处理或在戊烷中超声处理去除副产物，之后对其进行电子显微镜检查、化学分析和酶活性测定。结果表明，磷脂酶C处理后存在甘油二酯，且其是NADH-细胞色素c还原酶的抑制剂。用脂肪酶处理去除甘油二酯后，可证明NADH-细胞色素c还原酶对磷脂有需求。
