Uysal H, Saxton J, Hemming F W
Department of Biochemistry, The University of Nottingham, UK.
Glycoconj J. 1997 Jun;14(4):439-47. doi: 10.1023/a:1018543316793.
Fibroblasts from skin and skin lesions of patients with tuberous sclerosis (TS) and from skin of normal individuals were grown in culture. ELISA showed that the spent medium of those derived from TS skin lesions contained significantly more fibronectin (FN) than spent medium from the other cells. Amino acid compositional analysis of the FN from TS and normal sources revealed no substantial differences. However the FN of fibroblasts from TS-skin lesions was shown by HPAEC to contain a two- to three-fold increased content of carbohydrate. The changed monosaccharide composition was consistent with an increased content of N- and O-linked glycans and with the former containing polylactosamine chains. Fibroblasts from a normal individual were shown to proliferate more slowly and to produce larger cells when grown on FN from a TS skin lesion compared to growth on FN from normal skin.
对结节性硬化症（TS）患者的皮肤及皮肤损伤处的成纤维细胞，以及正常个体皮肤的成纤维细胞进行了体外培养。酶联免疫吸附测定（ELISA）显示，源自TS皮肤损伤处的成纤维细胞的用过的培养基中所含的纤连蛋白（FN）明显多于其他细胞的用过的培养基。对源自TS和正常来源的FN进行氨基酸组成分析，未发现实质性差异。然而，高效阴离子交换色谱（HPAEC）显示，来自TS皮肤损伤处的成纤维细胞的FN所含碳水化合物的含量增加了两到三倍。单糖组成的变化与N-连接和O-连接聚糖含量的增加一致，且前者含有多乳糖胺链。结果显示，与在源自正常皮肤的FN上生长相比，正常个体的成纤维细胞在源自TS皮肤损伤处的FN上生长时增殖更慢，且产生的细胞更大。
