野蕉花轴的多糖成分：水溶性多糖成分的主要结构特征

Polysaccharide components from the scape of Musa paradisiaca: main structural features of water-soluble polysaccharide component.

作者信息

Anjaneyalu Y V, Jagadish R L, Raju T S

机构信息

Department of Studies in Chemistry, University of Mysore Manasagangotri, India.

出版信息

Glycoconj J. 1997 Jun;14(4):507-12. doi: 10.1023/a:1018563821336.

DOI:10.1023/a:1018563821336

PMID:9249151

Abstract

Polysaccharide components present in the pseudo-stem (scape) of M. paradisiaca were purified from acetone powder of the scape by delignification followed by extraction with aqueous solvents into water soluble polysaccharide (WSP), EDTA-soluble polysaccharide (EDTA-SP), alkali-soluble polysaccharide (ASP) and alkali-insoluble polysaccharide (AISP) fractions. Sugar compositional analysis showed that WSP and EDTA-SP contained only D-Glc whereas ASP contained D-Glc, L-Ara and D-Xyl in approximately 1:1:10 ratio, respectively, and AISP contained D-Glc, L-Ara and D-Xyl in approximately 10:1:2 ratio, respectively. WSP was further purified by complexation with iso-amylalcohol and characterized by specific rotation, IR spectroscopy, Iodine affinity, ferricyanide number, blue value, hydrolysis with alpha-amylase and glucoamylase, and methylation linkage analysis, and shown to be a amylopectin type alpha-D-glucan.

摘要

从天堂蕉假茎（花葶）中存在的多糖成分，通过脱木质素从花葶的丙酮粉中进行纯化，随后用水性溶剂萃取，得到水溶性多糖（WSP）、EDTA 可溶性多糖（EDTA-SP）、碱溶性多糖（ASP）和碱不溶性多糖（AISP）组分。糖组成分析表明，WSP 和 EDTA-SP 仅含有 D-葡萄糖，而 ASP 分别含有比例约为 1:1:10 的 D-葡萄糖、L-阿拉伯糖和 D-木糖，AISP 分别含有比例约为 10:1:2 的 D-葡萄糖、L-阿拉伯糖和 D-木糖。WSP 通过与异戊醇络合进一步纯化，并通过比旋度、红外光谱、碘亲和力、铁氰化物值、蓝色值、用α-淀粉酶和糖化酶水解以及甲基化连接分析进行表征，结果表明它是一种支链淀粉型α-D-葡聚糖。