成肌细胞系L6的乙酰胆碱受体

Acetylcholine receptor of a myogenic cell line, L6.

作者信息

Seto A, Arimatsu Y, Amano T

出版信息

J Biochem. 1977 Oct;82(4):1019-24. doi: 10.1093/oxfordjournals.jbchem.a131772.

DOI:10.1093/oxfordjournals.jbchem.a131772

Abstract

The biochemical characters of acetylcholine receptor (AChR) in a myogenic cell line, L6, are as follows. 1. AChR extracted with Triton X-100 has a molecular weight of 5.5 X 10(5) determined by gel-chromatography, an apparent S value of 9.7 determined by sucrose density gradient centrifugation, and an isoelectric point of 5.3. AChR is shown to interact with concanavalin A. 2. The association rate constant of the binding reaction between AChR and alpha-bungarotoxin (alpha-BGT) labelled with 125I is 1 X 10(6) M-1 with S-1 at 35 degrees C. Preincubation with d-tubocurarine blocks the binding. The half-life of the AChR-toxin complex exceeds 180 h.

摘要

一种成肌细胞系L6中乙酰胆碱受体（AChR）的生化特性如下。1. 用Triton X - 100提取的AChR，通过凝胶色谱法测定其分子量为5.5×10⁵，通过蔗糖密度梯度离心法测定其表观S值为9.7，等电点为5.3。已证明AChR与伴刀豆球蛋白A相互作用。2. 在35℃下，AChR与用¹²⁵I标记的α - 银环蛇毒素（α - BGT）结合反应的缔合速率常数为1×10⁶ M⁻¹·s⁻¹。与d - 筒箭毒碱预孵育会阻断这种结合。AChR - 毒素复合物的半衰期超过180小时。

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