Crüger D G, Bruun-Petersen G, Kølvraa S
Department of Clinical Genetics, County Hospital of Vejle, Denmark.
Prenat Diagn. 1997 Jul;17(7):635-42. doi: 10.1002/(sici)1097-0223(199707)17:7<635::aid-pd127>3.0.co;2-2.
Uncultured coelomic cells were hybridized with alpha satellite DNA probes representing chromosomes X, Y, 18, and 13/21 in order to evaluate the distribution of hybridization signals obtained by fluorescence in situ-hybridization (FISH) analysis of this cell type. Cells from 26 samples were hybridized with the X probe and the Y probe was hybridized with cells from 25 of the samples. Cells from 16 and 11 samples were hybridized with an 18 alpha satellite DNA probe and 13/21 alpha satellite probe, respectively. The evaluation demonstrated that FISH with X, Y, 18, and 13/21 alpha satellite DNA probes on uncultured coelomic cells is a reliable technique since the distribution of hybridization signals is comparable to that seen in uncultured amniotic fluid cells.
将未培养的体腔细胞与代表X、Y、18和13/21号染色体的α卫星DNA探针进行杂交，以评估通过荧光原位杂交（FISH）分析该细胞类型所获得的杂交信号分布。来自26个样本的细胞与X探针杂交，Y探针与25个样本的细胞杂交。分别有16个和11个样本的细胞与18号α卫星DNA探针和13/21号α卫星探针杂交。评估表明，对未培养的体腔细胞进行X、Y、18和13/21号α卫星DNA探针的FISH是一种可靠的技术，因为杂交信号的分布与未培养的羊水细胞中所见的分布相当。
