Arnold A M, Peralta J M, Thonney M L
Department of Animal Science, Cornell University, Ithaca, NY 14853-4801, USA.
J Anim Sci. 1997 Jun;75(6):1495-503. doi: 10.2527/1997.7561495x.
Growth, nucleic acid, and protein concentrations were measured in three muscles of 20 rams, 20 wethers, and 20 wethers implanted with testosterone. Two lambs from each group were slaughtered at 14-d intervals from 49 to 133 d, and then at 28-d intervals until 217 d, for a total of 10 slaughter ages. Immediately after slaughter, the semitendinosus, splenius, and triceps brachii muscles were removed, trimmed of adhering fat, and weighed. The DNA, RNA, and protein concentrations of these muscles were determined. Testosterone increased combined weight of the three muscles. The splenius muscles of rams and wethers implanted with testosterone were heavier and had a biphasic growth pattern as the combined muscle weight increased, whereas the splenius muscle of wethers had a single growth phase. Rams and implanted wethers had greater splenius muscle DNA and RNA concentrations than wethers as muscle weight increased. This model could be used to study the gene regulation of testosterone-induced muscle growth with the possibility of invoking similar effects in more economically important muscles.
对20只公羊、20只阉羊以及20只植入睾酮的阉羊的三块肌肉进行了生长、核酸和蛋白质浓度的测定。从49日龄至133日龄，每隔14天从每组中宰杀2只羔羊，然后从133日龄至217日龄，每隔28天宰杀2只羔羊，总共10个屠宰年龄。屠宰后立即取出半腱肌、夹肌和肱三头肌，去除附着的脂肪并称重。测定这些肌肉的DNA、RNA和蛋白质浓度。睾酮增加了三块肌肉的总重量。植入睾酮的公羊和阉羊的夹肌较重，随着肌肉总重量的增加呈现双相生长模式，而阉羊的夹肌只有一个生长阶段。随着肌肉重量增加，公羊和植入睾酮的阉羊的夹肌DNA和RNA浓度高于阉羊。该模型可用于研究睾酮诱导肌肉生长的基因调控，并有可能在更具经济重要性的肌肉中引发类似效应。
