Lammoglia M A, Willard S T, Hallford D M, Randel R D
Texas A&M University, Texas Agricultural Experiment Station, Overton 75684, USA.
J Anim Sci. 1997 Jun;75(6):1591-600. doi: 10.2527/1997.7561591x.
Brahman cows were used to evaluate dietary fat (3.74% [control] and 5.20% [treated]) effects on blood hormone and lipid concentrations, follicular populations, and in vitro steroidogenesis. Cows were fed and ovaries were monitored by ultrasound from d 1 of the first estrous cycle (EC) until the first follicle of the second EC reached 8 mm, at which time ovaries were harvested. Follicular fluid (FF) was collected from large- and medium-sized follicles and assayed for progesterone (P4), estradiol-17 beta (E2), testosterone, cholesterol, and triglycerides. The corpus luteum was removed, minced, treated with LH, and incubated for 4 h. Granulosa cells harvested from the largest follicle were treated with forskolin, LH, or FSH and incubated for 48 h. Blood was collected during the treatment period and plasma assayed for 13,14-dihydro-15-keto-prostaglandin F(2 alpha) (PGFM), growth hormone (GH), insulin, P4 and E2. Cholesterol and triglycerides were assayed only from blood samples collected every 7 d. Treated cows had greater (P < .01) plasma E2 during the first EC and greater (P < .01) P4 during the second EC than controls. Treated cows had elevated (P < .01) insulin following d 16 of treatment, but GH and triglycerides were similar (P > .10) in both treatment groups. Treated cows tended (P = .10) to have greater PGFM peak concentrations than controls. Plasma cholesterol was elevated (P < .01) in treated cows on d 7 of the first EC. Treated cows tended to have greater (P < .09) follicular populations during the ovulatory and first wave of the first and second EC. Treatment did not affect (P > .10) FF concentrations of P4, E2, testosterone, cholesterol, or triglyceride from either large- or medium-sized follicles. There were no differences (P > .10) in steroid hormones produced in vitro.
婆罗门牛被用于评估日粮脂肪（3.74%[对照组]和5.20%[处理组]）对血液激素和脂质浓度、卵泡数量以及体外类固醇生成的影响。从第一个发情周期（EC）的第1天开始，对奶牛进行饲喂，并通过超声监测其卵巢，直到第二个EC的第一个卵泡达到8毫米，此时采集卵巢。从大、中卵泡中收集卵泡液（FF），并检测其中的孕酮（P4）、雌二醇-17β（E2）、睾酮、胆固醇和甘油三酯。取出黄体，切碎，用促黄体生成素（LH）处理，并孵育4小时。从最大卵泡中采集的颗粒细胞用福司可林、LH或促卵泡生成素（FSH）处理，并孵育48小时。在处理期间采集血液，检测血浆中的13,14-二氢-15-酮-前列腺素F（2α）（PGFM）、生长激素（GH）、胰岛素、P4和E2。仅对每7天采集的血液样本检测胆固醇和甘油三酯。与对照组相比，处理组奶牛在第一个EC期间血浆E2水平更高（P <.01），在第二个EC期间P4水平更高（P <.01）。处理组奶牛在处理第16天后胰岛素水平升高（P <.01），但两个处理组的GH和甘油三酯水平相似（P >.10）。处理组奶牛的PGFM峰值浓度有高于对照组的趋势（P =.10）。在第一个EC的第7天，处理组奶牛的血浆胆固醇水平升高（P <.01）。在第一个和第二个EC的排卵期及第一波期间，处理组奶牛的卵泡数量有多于对照组的趋势（P <.09）。处理对大、中卵泡中P4、E2、睾酮、胆固醇或甘油三酯的FF浓度没有影响（P >.10）。体外产生的类固醇激素没有差异（P >.10）。
