Korth R M
Forschung in der Allgemeinmedizin FIDA, Munich, Germany.
Int Arch Allergy Immunol. 1997 Aug;113(4):460-4. doi: 10.1159/000237623.
Infection, inflammation and allergy are characterized by an infiltration of neutrophils or eosinophils in the tissue and are associated with an increased level of lysophospholipids. In this study it is shown that labeled 1-O-alkyl-sn-glyceryl-3-phosphorylcholine (lyso-paf) binds to intact human blood neutrophils. Unlabeled lyso-paf(500 nM) inhibits the binding of [3H]lyso-paf to neutrophils in the presence of fatty acid-free serum albumin (0.25%). A linear Scatchard plot analysis of the specific [3H]lyso-paf binding to neutrophils revealed a KD value of 9.2 nM with 4,100 lyso-paf binding sites per neutrophil at 4 degrees C. Lyso-paf increased the specific binding of labeled platelet-activating factor ([3H]paf) to neutrophils at 20 degrees C. The increased specific binding of labeled paf to neutrophils could only be demonstrated when sterile cell preparation methods were used. Intact human blood eosinophils did not express specific lyso-paf binding sites. These results suggest that the lyso-paf binding sites on neutrophils have an up-regulatory function for paf which might be involved in neutrophil-mediated disorders.
感染、炎症和过敏的特征是组织中嗜中性粒细胞或嗜酸性粒细胞浸润，并且与溶血磷脂水平升高有关。本研究表明，标记的1-O-烷基-sn-甘油-3-磷酰胆碱（溶血血小板活化因子）可与完整的人血嗜中性粒细胞结合。在无脂肪酸血清白蛋白（0.25%）存在的情况下，未标记的溶血血小板活化因子（500 nM）可抑制[3H]溶血血小板活化因子与嗜中性粒细胞的结合。对[3H]溶血血小板活化因子与嗜中性粒细胞的特异性结合进行线性Scatchard图分析显示，在4℃时，KD值为9.2 nM，每个嗜中性粒细胞有4100个溶血血小板活化因子结合位点。溶血血小板活化因子在20℃时增加了标记的血小板活化因子（[3H]血小板活化因子）与嗜中性粒细胞的特异性结合。只有当使用无菌细胞制备方法时，才能证明标记的血小板活化因子与嗜中性粒细胞的特异性结合增加。完整的人血嗜酸性粒细胞不表达特异性溶血血小板活化因子结合位点。这些结果表明，嗜中性粒细胞上的溶血血小板活化因子结合位点对血小板活化因子具有上调功能，这可能与嗜中性粒细胞介导的疾病有关。
