Acevedo C G, Huambachano A, Perez E, Rojas S, Bravo I, Contreras E
Department of Physiology, Faculty of Biological Sciences, University of Conception, Concepcion, Chile.
Placenta. 1997 Jul-Aug;18(5-6):387-92. doi: 10.1016/s0143-4004(97)80038-0.
It has been suggested that adenosine is involved in the acute effects of ethanol in a number of tissues. The present study was undertaken to evaluate the role of adenosine on the vascular responses of perfused isolated human placental cotyledons after the acute administration of ethanol. The possibility that ethanol may effect the uptake and metabolism adenosine was also investigated. Uptake of adenosine was studied using the single-circulation paired-tracer dilution technique. Both adenosine and ethanol caused a dose-related increase in perfusion pressure of placental lobules. Pharmacologically relevant concentrations of ethanol (10-65 mM) significantly inhibited the uptake of [3H]adenosine between 25 and 50 per cent. Thin-layer chromatographic analysis of the perfusate after the administration of ethanol showed in a 17.9 +/- 0.6 per cent reduction of [3H]adenosine metabolism. These findings support the working hypothesis that placental adenosine, at least partially, mediates the placental disturbance elicited by the administration of acute ethanol, which may contribute to the pathogenesis of fetal alcohol syndrome.
有人提出，腺苷在许多组织中参与乙醇的急性效应。本研究旨在评估急性给予乙醇后，腺苷对灌注离体人胎盘小叶血管反应的作用。还研究了乙醇可能影响腺苷摄取和代谢的可能性。使用单循环双示踪剂稀释技术研究腺苷的摄取。腺苷和乙醇均导致胎盘小叶灌注压呈剂量相关增加。药理学相关浓度的乙醇（10 - 65 mM）显著抑制[3H]腺苷摄取25%至50%。给予乙醇后对灌注液进行的薄层色谱分析显示，[3H]腺苷代谢降低了17.9 +/- 0.6%。这些发现支持了这样一个工作假设，即胎盘腺苷至少部分介导了急性给予乙醇引起的胎盘功能紊乱，这可能有助于胎儿酒精综合征的发病机制。
