Wassarman P M, Ukena T E, Josefowicz W J, Letourneau G E, Karnovsky M J
J Cell Sci. 1977 Aug;26:323-37. doi: 10.1242/jcs.26.1.323.
Mouse oocytes are induced by cytochalasin B to undergo 'pseudocleavage' in vitro into 2 compartments, only one of which possesses microvilli. It has been found that this particular response to cytochalasin B is related to oocyte size and, possibly, to the acquisition of meiotic competence by the oocyte during its growth phase. Certain of the morphological events which characterize pseudocleavage have been determined using transmission and scanning electron microscopy. These events include: (i) an initial withdrawal of microvilli from the surface of the oocyte, together with the concomitant disappearance of microfilaments normally associated with the microvilli; (ii) the subsequent formation of a pseudocleavage furrow and contractile ring; and (iii) the reappearance of microvilli and associated microfilaments in one of the two resulting oocyte compartments. These changes in surface architecture are reflected in the distribution of fluorescein-conjugated lectins bound to the oocyte surface during pseudocleavage.
用细胞松弛素B诱导小鼠卵母细胞在体外进行“假分裂”，形成两个区室，其中只有一个具有微绒毛。已发现这种对细胞松弛素B的特殊反应与卵母细胞大小有关，并且可能与卵母细胞在其生长阶段获得减数分裂能力有关。使用透射电子显微镜和扫描电子显微镜确定了表征假分裂的某些形态学事件。这些事件包括：(i) 微绒毛最初从卵母细胞表面缩回，同时通常与微绒毛相关的微丝消失；(ii) 随后形成假分裂沟和收缩环；(iii) 在两个产生的卵母细胞区室之一中微绒毛和相关微丝重新出现。这些表面结构的变化反映在假分裂期间与卵母细胞表面结合的荧光素偶联凝集素的分布中。
