MacNeill S A, Fantes P A
Institute of Cell and Molecular Biology, University of Edinburgh, United Kingdom.
Methods Enzymol. 1997;283:440-59. doi: 10.1016/s0076-6879(97)83036-4.
Studies on DNA replication in S. pombe have provided powerful insights into the way in which the genome of this model eukaryote is replicated and how the replication process is controlled. These studies have been facilitated by the simplicity and range of methods available in this organism for physiological and genetic analysis of DNA replication mutants. In the future, continued focus on the analysis of such mutants, coupled with increasingly sophisticated biochemical investigation of the processes of DNA replication in both wild-type and mutant cells, will ensure continued rapid progress in this area.
对粟酒裂殖酵母DNA复制的研究为深入了解这种模式真核生物基因组的复制方式以及复制过程如何受到控制提供了有力的见解。该生物体中可用于DNA复制突变体生理和遗传分析的方法简单且多样，这推动了这些研究的开展。未来，持续关注此类突变体的分析，并结合对野生型和突变体细胞中DNA复制过程日益复杂的生化研究，将确保该领域继续取得快速进展。
