Hoshika Y
J Chromatogr. 1977 Dec 1;144(2):181-9. doi: 10.1016/s0021-9673(00)99353-1.
The simultaneous gas chromatographic separation of a mixture of 14 lower fatty acis, 11 phenols and 7 indoles has been effected by using a glass capillary column. Complete separation of the mixture has been obtained, except for phenol and o-cresol and o-ethylphenol and 3,5-xylenol whose peaks overlapped, and 2- and 3-methylindoles which were poorly separated. The optimum conditions are as follows: stationary phase, FFAP or PEG-20M; column (20 m X 0.28 mm I.D.) temperature, held for 1 min at 145 degrees (FFAP) or 130 degrees (PEG-20M); column oven, heated at 4 degrees/min from 145 or 130 to 180 degrees (FFAP) or 165 degrees (PEG-20M), maintained at 180 or 165 degrees for 30 min; carrier gas (helium) flow-rate, 0.6 ml/min (FFAP), 1.3 ml/min (PEG-20M); flame ionization detector. The method has been applied to the analysis of lower fatty acids, phenols and indoles in Japanese cigarette smoke, where the peaks were identified by the disappearance method using an alkaline pre-column.
使用玻璃毛细管柱实现了对14种低级脂肪酸、11种酚类和7种吲哚混合物的同时气相色谱分离。除了苯酚和邻甲酚、邻乙酚和3,5-二甲酚的峰重叠，以及2-甲基吲哚和3-甲基吲哚分离不佳外，已实现混合物的完全分离。最佳条件如下：固定相，FFAP或PEG-20M；柱（20 m×0.28 mm内径）温度，在145℃（FFAP）或130℃（PEG-20M）保持1分钟；柱箱，以4℃/分钟从145或130℃加热至180℃（FFAP）或165℃（PEG-20M），在180或165℃保持30分钟；载气（氦气）流速，0.6 ml/分钟（FFAP），1.3 ml/分钟（PEG-20M）；火焰离子化检测器。该方法已应用于日本香烟烟雾中低级脂肪酸、酚类和吲哚的分析，其中峰通过使用碱性预柱的消失法进行鉴定。
