Namera A, Yashiki M, Nagasawa N, Iwasaki Y, Kojima T
Department of Legal Medicine, Hiroshima University School of Medicine, Japan.
Forensic Sci Int. 1997 Aug 4;88(2):125-31. doi: 10.1016/s0379-0738(97)00062-5.
A simple and rapid method for analysis of malathion in blood was developed using head space-solid phase microextraction (HS-SPME) and gas chromatography mass spectrometry/ electron impact ionization-selected ion monitoring (GC-MS/EI-SIM). A vial containing a blood sample, ammonium sulphate, sulphuric acid and fenitrothion as an internal standard, was heated at 90 degrees C for 15 min. The extraction fiber of the SPME was exposed for 5 min in the head space of the vial. The compounds absorbed on the fiber were detached by exposing the fibre in the injection port of GC-MS. A straight calibration curve was obtained between malathion concentrations of 2.5 to 50.0 micrograms g-1 in blood. No interfering substances were found, and the time for analysis was 40 min for one sample.
采用顶空-固相微萃取（HS-SPME）和气相色谱-质谱联用/电子轰击电离-选择离子监测（GC-MS/EI-SIM）技术，开发了一种简单快速的血液中马拉硫磷分析方法。将含有血样、硫酸铵、硫酸和作为内标的杀螟硫磷的小瓶在90℃加热15分钟。SPME的萃取纤维在小瓶顶空中暴露5分钟。通过将纤维暴露在GC-MS的进样口中，使吸附在纤维上的化合物解吸。在血液中马拉硫磷浓度为2.5至50.0微克/克之间获得了一条直线校准曲线。未发现干扰物质，一个样品的分析时间为40分钟。
