Koo G C, Goldberg C L, Shen F W
J Exp Med. 1979 Oct 1;150(4):1028-32. doi: 10.1084/jem.150.4.1028.
Assays of H-Y-specific cell-mediated cytolysis (CMC) in vitro were carried out with B6 female effector cells and B6 male target cells. This lytic system depends on compatibility for H-2Db but not for H-2Kb. Antibody to H-2Db but not to H-2Kb blocked the CMC assay. However, maximal blocking required exposure to the target cells to H-2Db antibody for up to 2 h. This interval is far longer than would be required for blocking of a pre-existing H-Y:H-2Db complex, and seems more consonant with a membrane reorganization bringing H-Y and H-2Db together, as proposed from mapping studies. The question whether the postulated configurational change affecting H-Y and H-2Db is a necessary feature of CMC awaits further study.
使用B6雌性效应细胞和B6雄性靶细胞进行了体外H - Y特异性细胞介导的细胞溶解（CMC）测定。这种溶解系统依赖于H - 2Db的相容性，但不依赖于H - 2Kb。抗H - 2Db而非抗H - 2Kb的抗体阻断了CMC测定。然而，最大阻断需要将靶细胞暴露于H - 2Db抗体长达2小时。这个间隔时间远比阻断预先存在的H - Y:H - 2Db复合物所需的时间长得多，并且似乎更符合从图谱研究中提出的使H - Y和H - 2Db聚集在一起的膜重组。影响H - Y和H - 2Db的假定构型变化是否是CMC的必要特征有待进一步研究。
