Lopez-Ocejo O, Herrera A, Herrera L, De la Fuente J
Center for Biological Research, Havana, Cuba.
Biol Res. 1995;28(4):283-90.
Analysis of gene expression in peripheral blood lymphocytes is of special interest because it could reflect physiological conditions. We have examined the expression and compared the relative amounts of specific mRNAs for interferons (IFN-alpha and IFN-beta), tumor necrosis factor-alpha (TNF-alpha) and interferon regulatory factors (IRF-1 and IRF-2) from interferon primed and Sendai virus induced peripheral blood leukocytes. Results obtained showed that IRF-1 was highly inducible by IFN treatment, IFN-alpha, TNF-alpha and IRF-2 were weakly induced by IFN treatment, and IFN-beta was not inducible by priming the cells with recombinant human IFN-alpha 2b. The IFN-alpha, IFN-beta, IRF-2 and TNF-alpha transcripts increased upon viral infection. The IRF-1 mRNA was rapidly induced by IFN treatment and decreased after Sendai virus infection. Our results show that, in peripheral blood lymphocytes, IFN-alpha and -beta genes have a different response to IFN induction, thus suggesting different regulatory mechanisms for IFN induction of type I IFN genes in peripheral blood lymphocytes.
外周血淋巴细胞中的基因表达分析具有特殊意义，因为它可以反映生理状况。我们检测了经干扰素预处理和仙台病毒诱导的外周血白细胞中干扰素（IFN-α和IFN-β）、肿瘤坏死因子-α（TNF-α）以及干扰素调节因子（IRF-1和IRF-2）的特定mRNA的表达，并比较了它们的相对含量。结果显示，IRF-1经IFN处理后高度可诱导，IFN-α、TNF-α和IRF-2经IFN处理后诱导较弱，用重组人IFN-α 2b预处理细胞后IFN-β不可诱导。病毒感染后，IFN-α、IFN-β、IRF-2和TNF-α转录本增加。IRF-1 mRNA经IFN处理后迅速诱导，在仙台病毒感染后减少。我们的结果表明，在外周血淋巴细胞中，IFN-α和-β基因对IFN诱导有不同反应，这表明外周血淋巴细胞中I型IFN基因的IFN诱导存在不同的调控机制。
