Batista M R, Ferraz M, Bazotte R B
Department of Pharmacy and Pharmacology (DFF), University of Maringá, PR, Brazil.
Physiol Behav. 1997 Aug;62(2):249-53. doi: 10.1016/s0031-9384(97)00110-8.
Rats trained to eat a single meal from 0800 to 1000 AM, (MF rats), increased food intake from the 1st (7.0 g) to the 13th (16.1 g) day and showed higher hepatic glycogen concentration and glycemia during fasting. To verify if these changes were determined by the higher food intake or due to the disciplining condition we compared rats at the 1st (MF(1st day-5 g) group) and 13th day (MF(13th day-5 g) group) of training, refed with a fixed amount of food (5 g). In addition, a third group (MF(13th day-12 g) group) composed by trained meal-fed rats, refed on day 13 with approximately 75% of food ingested by MF rats on day 13 ( 12 g) of feeding training was included. The experiments with these 3 groups were performed at zero (1000 h), 6 (1600 h), 13 (2300 h), 18 (0400 h) and 22 (0800 h) h after meal. Our results demonstrated that part of the physiological changes of MF rats is consequence of feeding training (increased food intake during the fixed meal time and spontaneous elevation of glycemia 13 h after meal) and part of the differences (hepatic glycogen concentration, gastric and intestinal emptying) correlate well with effective time of fasting. In addition, hepatic gluconeogenesis from L-lactate and glycerol was influenced by both factors.
训练在上午8点到10点进食一餐的大鼠（MF大鼠），从第1天（7.0克）到第13天（16.1克）食物摄入量增加，并且在禁食期间肝糖原浓度和血糖水平更高。为了验证这些变化是由更高的食物摄入量还是由进食规律条件所决定，我们比较了训练第1天（MF(第1天 - 5克)组）和第13天（MF(第13天 - 5克)组）的大鼠，给它们重新投喂固定量的食物（5克）。此外，还纳入了第三组（MF(第13天 - 12克)组），该组由经过训练的定时进食大鼠组成，在第13天重新投喂的食物量约为MF大鼠在进食训练第13天摄入食物量的75%（12克）。对这三组大鼠的实验在进食后0（1000时）、6（1600时）、13（2300时）、18（0400时）和22（0800时）小时进行。我们的结果表明，MF大鼠的部分生理变化是进食训练的结果（在固定进餐时间内食物摄入量增加以及餐后13小时血糖自发升高），部分差异（肝糖原浓度、胃和肠道排空）与有效禁食时间密切相关。此外，L - 乳酸和甘油的肝糖异生受这两个因素的影响。
