Bianchi L, Colivicchi M A, Della Corte L, Valoti M, Sgaragli G P, Bechi P
Dipartimento di Farmacologia Preclinica e Clinica M. Aiazzi Mancini, Università degli Studi di Firenze, Italy.
J Chromatogr B Biomed Sci Appl. 1997 Jul 4;694(2):359-65. doi: 10.1016/s0378-4347(97)00150-3.
The antioxidants, 2-tert.-butyl-4-methoxyphenol (BHA) and its oxidative peroxidation product 2,2'-dihydroxy-3,3'-di-tert.-butyl-5,5'-dimethoxybiphenyl (di-BHA), 3,5-di-tert.-butyl-4-hydroxytoluene (BHT) and propyl gallate, were measured in plasma and tissue homogenates by HPLC and electrochemical detection, with a sensitivity down to 0.2 (BHA), 0.1 (di-BHA), 0.4 (BHT) and 1 (propyl gallate) ng ml(-1) of plasma or tissue homogenate. The data demonstrate that in man, at the current level of exposure to dietary antioxidants, significant amounts of BHA, BHT and propyl gallate are accumulated in the omentum. Furthermore, they provide the first evidence that the peroxidase-catalysed oxidation of BHA is operative in man.
通过高效液相色谱法和电化学检测法测定血浆和组织匀浆中的抗氧化剂，2-叔丁基-4-甲氧基苯酚（丁基羟基茴香醚，BHA）及其氧化过氧化产物2,2'-二羟基-3,3'-二叔丁基-5,5'-二甲氧基联苯（二丁基羟基茴香醚，di-BHA）、3,5-二叔丁基-4-羟基甲苯（二丁基羟基甲苯，BHT）和没食子酸丙酯，血浆或组织匀浆的检测灵敏度低至0.2（BHA）、0.1（di-BHA）、0.4（BHT）和1（没食子酸丙酯）纳克/毫升。数据表明，在人类中，在目前饮食抗氧化剂的暴露水平下，大网膜中会积累大量的BHA、BHT和没食子酸丙酯。此外，这些数据首次证明了BHA的过氧化物酶催化氧化在人类中起作用。
