压力刺激大鼠肠道上皮细胞的增殖和DNA合成。

Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells.

作者信息

Hirokawa M, Miura S, Shigematsu T, Yoshida H, Hokari R, Higuchi H, Kurose I, Kimura H, Saito H, Nakaki T, Ishii H

机构信息

Department of Internal Medicine, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan.

出版信息

Life Sci. 1997;61(7):667-72. doi: 10.1016/s0024-3205(97)00531-6.

DOI:10.1016/s0024-3205(97)00531-6

PMID:9252241

Abstract

Effects of intraluminal pressure on cultured intestinal epithelial cells were assessed by measuring cell proliferation and DNA synthesis after exposure to various pressures. Pressures of 40 to 120 mm Hg promoted cell proliferation and DNA synthesis. Pressure-induced DNA synthesis was further enhanced by the addition of interleukin-2, suggesting the regulation of intestinal epithelial growth by pressure in coordination with cytokines. Pretreatment with either a phospholipase C inhibitor or protein kinase C inhibitor significantly inhibited DNA synthesis promoted by pressure and interleukin-2. This study demonstrates a novel mechanism whereby pressure regulates cell growth in intestinal epithelial cells, possibly via activation of phospholipase C and protein kinase C.

摘要

通过测量暴露于不同压力后细胞增殖和DNA合成情况，评估管腔内压力对培养的肠上皮细胞的影响。40至120毫米汞柱的压力可促进细胞增殖和DNA合成。添加白细胞介素-2可进一步增强压力诱导的DNA合成，这表明压力与细胞因子协同调节肠上皮生长。用磷脂酶C抑制剂或蛋白激酶C抑制剂预处理可显著抑制压力和白细胞介素-2促进的DNA合成。本研究证明了一种新机制，即压力可能通过激活磷脂酶C和蛋白激酶C来调节肠上皮细胞的生长。