Gerhart D Z, Enerson B E, Zhdankina O Y, Leino R L, Drewes L R
Department of Biochemistry, University of Minnesota, Duluth 55812, USA.
Am J Physiol. 1997 Jul;273(1 Pt 1):E207-13. doi: 10.1152/ajpendo.1997.273.1.E207.
A polyclonal affinity-purified antibody to the carboxyl-terminal end of the rat monocarboxylate transporter 1 (MCT1) was generated in chickens and used in immunocytochemical studies of brain tissue sections from adult and suckling rats. The antibody identified a 48-kDa band on immunoblots and stained tissue sections of heart, cecum, kidney, and skeletal muscle, consistent with the reported molecular mass and cellular expression for this transporter. In tissue sections from adult brains, the antibody labeled brain microvessel endothelial cells, ependymocytes, glial-limiting membranes, and neuropil. In brain sections from 3- to 14-day-old rats, microvessels were much more strongly labeled and neuropil was weakly labeled compared with sections from adults. Immunoelectron microscopy indicated that labeling was present on both luminal and abluminal endothelial cell plasma membranes. These results suggest that MCT1 may play an important role in the passage of lactate and other monocarboxylates across the blood-brain barrier and that suckling rats may be especially dependent on this transporter to supply energy substrates to the brain.
在鸡体内产生了一种针对大鼠单羧酸转运蛋白1（MCT1）羧基末端的多克隆亲和纯化抗体，并将其用于成年和乳鼠脑组织切片的免疫细胞化学研究。该抗体在免疫印迹上识别出一条48 kDa的条带，并对心脏、盲肠、肾脏和骨骼肌的组织切片进行染色，这与该转运蛋白报道的分子量和细胞表达情况一致。在成年大鼠脑组织切片中，该抗体标记了脑微血管内皮细胞、室管膜细胞、胶质界膜和神经毡。在3至14日龄大鼠的脑切片中，与成年大鼠的切片相比，微血管的标记要强得多，而神经毡的标记较弱。免疫电子显微镜显示，标记物存在于管腔和管腔外的内皮细胞质膜上。这些结果表明，MCT1可能在乳酸和其他单羧酸跨血脑屏障的转运中起重要作用，并且乳鼠可能特别依赖这种转运蛋白为大脑提供能量底物。
