Fernández-Abalos J M, Ruiz-Arribas A, Garda A L, Santamaría R I
Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Cientificas (CSIC)/Universidad de Salamanca, Spain.
FEMS Microbiol Lett. 1997 Aug 1;153(1):97-103. doi: 10.1111/j.1574-6968.1997.tb10469.x.
The production of the cellulase Cell from Streptomyces halstedii JM8 was studied in cells grown in the presence of glucose, cellobiose or microcrystalline cellulose (Avicel). Among these, glucose repressed expression while cellobiose and Avicel exerted a clear induction. The gene celA1 was cloned in several heterologous Streptomyces hosts and its expression analyzed. S. parvulus transformed with the plasmid pJM11, a pIJ702 derivative, was the best producer. A region which includes the sequence ATTGGGACCGCTTCC located between positions -161 and -147 upstream from the translation initiation codon [Fernández-Abalos et al. (1992) J. Bacteriol. 174, 6368-6376] was deleted and its effect was studied in the presence of different carbon sources. Although the observed effect depends on the host used, this region seems to be involved in activation of the expression of this gene.
对哈氏链霉菌JM8产生纤维素酶Cell的情况进行了研究，研究对象是在葡萄糖、纤维二糖或微晶纤维素（微晶纤维素）存在下生长的细胞。其中，葡萄糖会抑制表达，而纤维二糖和微晶纤维素则有明显的诱导作用。将celA1基因克隆到几种异源链霉菌宿主中并分析其表达情况。用质粒pJM11（pIJ702的衍生物）转化的小链霉菌是最佳产生菌。对翻译起始密码子上游-161至-147位之间包含序列ATTGGGACCGCTTCC的区域进行了缺失，并研究了其在不同碳源存在下的作用。尽管观察到的效果取决于所使用的宿主，但该区域似乎参与了该基因表达的激活。
