Nair S A, Nair M B, Jayaprakash P G, Rajalekshmy T N, Nair M K, Pillai M R
Division of Laboratory Medicine, Regional Cancer Centre, Thiruvananthapuram, India.
Pathobiology. 1997;65(2):100-7. doi: 10.1159/000164110.
The expression of cytokeratins (CKs) in normal cervical epithelium, low grade squamous intraepithelial lesions (SIL), high grade SILs and squamous cell carcinoma (SCC) were analyzed using four different monoclonal antikeratin antibodies. In normal cervical epithelium, CK 18 showed strong immunoreactivity in basal and parabasal layers. CK 19 and 14 were expressed only in the basal layer while CK 13 was found selectively n the spinal cells. As the lesions progressed from low grade SIL to high grade SIL, immunoreactivity of CK 18, 19 and 14 in the basal cell compartment increased while the expression of CK 13 decreased. In SCC, as well-differentiated tumors showed decreased immunoreactivity for CK 18, 19 and 14 with CK 13 showing a strong and focal (localized) immunoreactivity. Undifferentiated carcinomas totally lacked CK 13 reactivity. Our findings therefore suggest that expression of CK 18, 19 and 14 may be directly related to tumor grade and CK 13 may be a marker of differentiation in cervical lesions.
使用四种不同的单克隆抗角蛋白抗体分析了细胞角蛋白（CKs）在正常宫颈上皮、低级别鳞状上皮内病变（SIL）、高级别SIL和鳞状细胞癌（SCC）中的表达情况。在正常宫颈上皮中，CK 18在基底层和副基底层显示出强免疫反应性。CK 19和14仅在基底层表达，而CK 13选择性地在棘细胞中发现。随着病变从低级别SIL进展到高级别SIL，基细胞区室中CK 18、19和14的免疫反应性增加，而CK 13的表达减少。在SCC中，高分化肿瘤对CK 18、19和14的免疫反应性降低，CK 13显示出强而局灶性（定位性）免疫反应性。未分化癌完全缺乏CK 13反应性。因此，我们的研究结果表明，CK 18、19和14的表达可能与肿瘤分级直接相关，而CK 13可能是宫颈病变中分化的标志物。
