Kataoka H
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
J Chromatogr A. 1997 Jul 11;774(1-2):121-42. doi: 10.1016/s0021-9673(97)00246-x.
The environmental analysis of heterocyclic amines is important to preserve human health, because all these compounds are potent mutagens and many are carcinogens in experimental animals. This review summarizes the analytical methods for the determination of mutagenic heterocyclic amines and their applications to various environmental samples. High-performance liquid chromatographic methods with ultraviolet, electrochemical and fluorescence detections are commonly used for the quantification of heterocyclic amines. Moreover, liquid chromatography-mass spectrometry, gas chromatography, gas chromatography-mass spectrometry, capillary zone electrophoresis and enzyme-linked immunosorbent assay are also developed. Heterocyclic amines in complex environmental matrices are present at low parts per billion or less, so that the sample preparation influences the reliable and accurate analysis of these compounds. Mutagenic heterocyclic amines are widely distributed in a number of ambient environmental components such as airbone particles, diesel-exhaust particles, cigarette smoke, cooking fumes, rain water, sewage water, incineration-ash and soil. These facts suggest that mutagenic heterocyclic amines are likely to be ubiquitous environmental pollutants and humans are continually exposed to these compounds in normal daily life.
杂环胺的环境分析对于保护人类健康至关重要，因为所有这些化合物都是强效诱变剂，并且许多在实验动物中具有致癌性。本综述总结了用于测定诱变杂环胺的分析方法及其在各种环境样品中的应用。具有紫外、电化学和荧光检测的高效液相色谱方法通常用于杂环胺的定量。此外，液相色谱-质谱、气相色谱、气相色谱-质谱、毛细管区带电泳和酶联免疫吸附测定也已得到发展。复杂环境基质中的杂环胺含量低至十亿分之几或更低，因此样品制备会影响这些化合物的可靠和准确分析。诱变杂环胺广泛分布于许多环境成分中，如空气颗粒物、柴油机尾气颗粒、香烟烟雾、烹饪油烟、雨水、污水、焚烧灰和土壤。这些事实表明，诱变杂环胺可能是普遍存在的环境污染物，人类在日常生活中持续接触这些化合物。
