Wien M W, Curry S, Filman D J, Hogle J M
Committee on Higher Degrees in Biophysics, Harvard University, Cambridge, Massachusetts 02138, USA.
Nat Struct Biol. 1997 Aug;4(8):666-74. doi: 10.1038/nsb0897-666.
In order to better understand the process of cell entry for non-enveloped viruses, we have solved the crystal structures of five poliovirus mutants which can infect cells expressing mutant poliovirus receptors. Four of these structures have been solved from frozen crystals using cryocrystallographic data collection methods. The mutations have a range of structural consequences, from small local perturbations to significant loop rearrangements. All of the mutant viruses are more labile to conversion to an apparent cell entry intermediate, suggesting that these mutant viruses could compensate for the suboptimal receptors by lowering the thermal energy required to undergo the receptor-mediated conformational change.
为了更好地理解无包膜病毒的细胞进入过程，我们解析了五种能够感染表达突变型脊髓灰质炎病毒受体的细胞的脊髓灰质炎病毒突变体的晶体结构。其中四种结构是使用低温晶体学数据收集方法从冷冻晶体中解析出来的。这些突变具有一系列结构后果，从小的局部扰动到显著的环重排。所有突变病毒转化为明显的细胞进入中间体时更不稳定，这表明这些突变病毒可以通过降低经历受体介导的构象变化所需的热能来补偿次优受体。
