Callebaut M, van Nueten E, Harrisson F, van Nassauw L, Schrevens A, Bortier H
Laboratory of Human Anatomy and Embryology, University of Antwerp (RUCA), Belgium.
Eur J Morphol. 1997 Apr;35(2):69-77. doi: 10.1076/ejom.35.2.69.13065.
In the present study, we removed the whole area marginalis, Rauber's sickle and the peripheral part of the area centralis from unincubated chicken blastoderms (st IV, Vakaet, 1962a). By placing a fragment of a quail Rauber's sickle (functioning as early gastrulation organizer: Callebaut and Van Nueten, 1994) at different places and oriented in different directions on the remaining central part of the area centralis, we observed, after in vitro culture, a normal embryonic development. This indicates that the area marginalis itself is not indispensable for gastrulation and neurulation. Our study also indicates that none of the three elementary tissues (Rauber's sickle, endophyll and upper layer) of the avian unincubated blastoderm present an irreversible functional polarity.
在本研究中，我们从未孵化的鸡胚盘（第IV期，瓦凯特，1962a）中移除了整个边缘区、劳伯氏新月区和中央区的周边部分。通过将鹌鹑劳伯氏新月区的一个片段（作为早期原肠胚形成组织者起作用：卡勒博和范纽滕，1994）放置在中央区剩余中央部分的不同位置并使其朝向不同方向，我们在体外培养后观察到了正常的胚胎发育。这表明边缘区本身对于原肠胚形成和神经胚形成并非不可或缺。我们的研究还表明，鸟类未孵化胚盘的三种基本组织（劳伯氏新月区、内胚层和上层）中没有一种呈现不可逆的功能极性。
