Weng S F, Tang S M, Tseng Y H, Lin J W
Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan, Republic of China.
DNA Seq. 1997;7(3-4):221-3. doi: 10.3109/10425179709034039.
An insertion sequence element, ISXC6, was isolated from Xanthomonas campestris pv. campestris 17 (Xc17). Sequence analysis showed that it is 1,500 bp long and has 20-bp perfect inverted repeat ends. Upon transposition, a direct repeat TAATTC was generated, flanking this IS. No significant homology was observed between this sequence and other sequences in database. Results of Southern hybridization showed that multiple copies of ISXC6 were present in 7 strains of Xanthomonas examined.
从野油菜黄单胞菌野油菜致病变种17(Xc17)中分离出一个插入序列元件ISXC6。序列分析表明,它长1500 bp,具有20 bp的完美反向重复末端。转座时,在该插入序列两侧产生了一个直接重复序列TAATTC。该序列与数据库中的其他序列未观察到显著同源性。Southern杂交结果表明,在所检测的7株黄单胞菌中存在多个ISXC6拷贝。