一种直接对黏粒LAWRIST16克隆进行测序的方法。

A method to direct sequence cosmid LAWRIST16 clones.

作者信息

Repetto M, Ballabio A, Zollo M

机构信息

Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milan, Italy.

出版信息

DNA Seq. 1997;7(3-4):229-33. doi: 10.3109/10425179709034041.

DOI:10.3109/10425179709034041

PMID:9254019

Abstract

We developed a new cycle condition method optimized for direct sequencing LAWRIST16 cosmid clones based on dye terminator and dye primer cycle sequencing chemistries. We report a direct comparison of the two most widely used sequencing polymerase enzymes (AmpliTaq FS and Thermosequenase). The sequencing data obtained is of high quality and we believe this method could be routinely used in large scale sequencing facilities.

摘要

我们基于染料终止剂和染料引物循环测序化学方法，开发了一种针对直接测序LAWRIST16黏粒克隆进行优化的新循环条件方法。我们报告了两种最广泛使用的测序聚合酶（AmpliTaq FS和Thermosequenase）的直接比较。获得的测序数据质量很高，我们相信这种方法可在大规模测序设施中常规使用。