Saland L C, Apodaca A, Ramirez D, Hernandez V, Gaddy J, Thomas D
Department of Anatomy, University of New Mexico School of Medicine, Albuquerque, USA.
Brain Res Bull. 1997;43(6):561-4. doi: 10.1016/s0361-9230(97)80005-6.
The density of synaptophysin (SN)-immunoreactivity (IR) was examined in pituitary glands of aging male Sprague-Dawley rats. SN-IR was observed as dense dots among endocrine cells of the intermediate lobe, while the neural lobe contained numerous, highly dense immunopositive regions. Some anterior lobe secretory cells contained SN-IR within the cytoplasm, suggestive of the presence of the protein in secretory granules, but no dot-like staining was observed between endocrine cells of that region. A quantitative analysis of the dot-like SN-immunostaining within the intermediate lobe found that tissue from groups of rats aged 13 months, or 15-17 months, contained significantly fewer SN-immunopositive areas than did tissues from 8-month-old animals. Diminished SN immunostaining is suggestive of reduced numbers of synapses in the intermediate lobe, which may lead to alterations in regulation of pituitary hormone secretion from endocrine cells in the older animals.
研究了衰老雄性斯普拉格-道利大鼠垂体中突触素（SN）免疫反应性（IR）的密度。在中间叶的内分泌细胞中，SN-IR表现为密集的小点，而神经叶则含有大量高度密集的免疫阳性区域。一些前叶分泌细胞的细胞质中含有SN-IR，提示该蛋白存在于分泌颗粒中，但在该区域的内分泌细胞之间未观察到点状染色。对中间叶内点状SN免疫染色的定量分析发现，13个月龄或15 - 17个月龄大鼠组的组织中，SN免疫阳性区域明显少于8个月龄动物的组织。SN免疫染色减弱提示中间叶突触数量减少，这可能导致老年动物内分泌细胞垂体激素分泌调节的改变。
