正常血脂和高甘油三酯血症受试者中载脂蛋白C-III的血浆脂蛋白分布：不同脂蛋白组分中载脂蛋白C-III与载脂蛋白E比值的比较

Plasma lipoprotein distribution of apoC-III in normolipidemic and hypertriglyceridemic subjects: comparison of the apoC-III to apoE ratio in different lipoprotein fractions.

作者信息

Fredenrich A, Giroux L M, Tremblay M, Krimbou L, Davignon J, Cohn J S

机构信息

Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montreal, Quebec, Canada.

出版信息

J Lipid Res. 1997 Jul;38(7):1421-32.

PMID:9254067

Abstract

In order to assess the relationship between plasma accumulation of triglyceride-rich lipoproteins (TRL) and lipoprotein levels of apoC-III and apoE, we have measured apoC-III and apoE in lipoproteins separated according to size (by automated gel filtration chromatography) from plasma of normolipidemic subjects (plasma triglyceride (TG): 0.84 +/- 0.10 mmol/l; mean +/- SE, n = 8), and from type III (n = 8) and type IV (n = 8) hyperlipoproteinemic patients, matched for plasma TG (5.76 +/- 0.62 v 5.55 +/- 0.45 mmol/l, resp.). Total plasma apoC-III concentration was similar in type III and type IV patients (33.1 +/- 3.4 v 37.6 +/- 4.4 mg/dl, respectively), but was significantly increased compared to normolipidemic controls (10.0 +/- 1.0 mg/dl, P < 0.001). TRL apoC-III was lower and high density lipoprotein (HDL) apoC-III was significantly higher in type III versus type IV subjects (14.8 +/- 3.2 vs. 22.8 +/- 3.0 mg/dl, P < 0.05; 8.3 +/- 1.0 vs. 5.2 +/- 0.5 mg/dl, P < 0.05). Plasma concentration of apoC-III in lipoproteins that eluted between TRL and HDL (intermediate-sized lipoproteins, ISL) was similar in the two hypertriglyceridemic groups (10.1 +/- 1.3 vs. 9.7 +/- 1.6 mg/dl), but was significantly higher (P< 0.05) than controls (2.2 +/- 0.3 mg/dl). TRL, ISL, and HDL apoE concentrations were significantly higher in type III versus type IV subjects (P < 0.05). All lipoprotein fractions in type III patients were characterized by lower apoC-III to apoE ratios. In contrast, the TRL apoC-III to apoE ratio of type IV patients was similar and the ISL apoC-III to apoE ratio was significantly higher, compared to normolipidemic individuals. These results indicate that compared to normolipidemic individuals, remnant-like lipoproteins in the ISL fraction of type IV patients are enriched in apoC-III relative to apoE, whereas those of type III patients are enriched in apoE relative to apoC-III.

摘要

为了评估富含甘油三酯的脂蛋白（TRL）的血浆蓄积与载脂蛋白C-III（apoC-III）和载脂蛋白E（apoE）的脂蛋白水平之间的关系，我们对血脂正常的受试者（血浆甘油三酯（TG）：0.84±0.10 mmol/l；平均值±标准误，n = 8）、III型（n = 8）和IV型（n = 8）高脂血症患者血浆中按大小分离的脂蛋白（通过自动凝胶过滤色谱法）中的apoC-III和apoE进行了测量，这些患者的血浆TG相匹配（分别为5.76±0.62和5.55±0.45 mmol/l）。III型和IV型患者的血浆总apoC-III浓度相似（分别为33.1±3.4和37.6±4.4 mg/dl），但与血脂正常的对照组（10.0±1.0 mg/dl）相比显著升高（P < 0.001）。与IV型受试者相比，III型受试者的TRL apoC-III较低，高密度脂蛋白（HDL）apoC-III显著较高（14.8±3.2对22.8±3.0 mg/dl，P < 0.05；8.3±1.0对5.2±0.5 mg/dl，P < 0.05）。在两个高甘油三酯血症组中，洗脱于TRL和HDL之间的脂蛋白（中等大小脂蛋白，ISL）中的apoC-III血浆浓度相似（10.1±1.3对9.7±1.6 mg/dl），但显著高于对照组（2.2±0.3 mg/dl，P < 0.05）。与IV型受试者相比，III型受试者的TRL、ISL和HDL apoE浓度显著更高（P < 0.05）。III型患者的所有脂蛋白组分的特点是apoC-III与apoE的比值较低。相比之下，与血脂正常的个体相比，IV型患者的TRL apoC-III与apoE的比值相似，而ISL apoC-III与apoE的比值显著更高。这些结果表明，与血脂正常的个体相比，IV型患者ISL组分中的残留样脂蛋白相对于apoE富含apoC-III，而III型患者的残留样脂蛋白相对于apoC-III富含apoE。