粟酒裂殖酵母基因组整合图谱。

Integrated map of the Schizosaccharomyces pombe genome.

作者信息

Garkavtsev I, Mizukami T

机构信息

Department of Medical Biochemistry and Southern Alberta Cancer Research Center, University of Calgary HSC, 3330 Hospital Dr. N.W., Calgary, Alberta, Canada T2N 4N1.

出版信息

Chromosoma. 1997 Sep;106(4):254-65. doi: 10.1007/s004120050246.

DOI:10.1007/s004120050246

PMID:9254727

Abstract

A restriction map of the entire Schizosaccharomyces pombe genome was constructed using two restriction enzymes (BamHI and PstI) that recognize 6 bp. The restriction map contains 420 minimally overlapping clones (miniset) and has 22 gaps. We located 126 genes, marker fragments of DNA (NotI and SfiI linking clones), and 36 transposable elements by hybridization to unique restriction fragments.

摘要

利用两种识别6个碱基对的限制性内切酶（BamHI和PstI）构建了粟酒裂殖酵母全基因组的限制性图谱。该限制性图谱包含420个最小重叠克隆（最小集），有22个缺口。我们通过与独特的限制性片段杂交定位了126个基因、DNA标记片段（NotI和SfiI连接克隆）以及36个转座元件。