Goupille P, Chevalier X, Valat J P, Garaud P, Perin F, Le Pape A
Department of Rheumatology, University Hospital, Tours, France.
Br J Rheumatol. 1997 Jul;36(7):758-62. doi: 10.1093/rheumatology/36.7.758.
The potential of scintigraphy with technetium 99m-labelled J001 (99mTc-J001) to detect synovitis was studied in 15 rabbits with osteoarthritis (OA) of the right knee (section of cruciate ligaments), in five sham-operated rabbits and in four non-operated rabbits. J001 is a non-pyrogenic, acylated poly (1,3) galactoside isolated from the membrane of a non-pathogenic strain of Klebsiella pneumoniae which is able to bind selectively to macrophages via the binding to CD11b and CD14 molecules. The results of 99mTc-J001 scintigraphy were compared with those of scintigraphy with 99mTc-labelled methylene diphosphonate (99mTc-MDP) and GC-APG (a derivative of J001 unable to bind macrophages in vitro). The mean scintigraphic ratios (diseased healthy knee) of 99mTc-J001 were significantly higher in OA rabbits than in sham- and non-operated rabbits, from as early as day 18 until day 90. 99mTc-J001 scintigraphy demonstrated earlier increased uptake than 99mTc-MDP scintigraphy. The mean scintigraphic ratios of 99mTc-J001 were significantly higher than those of 99mTc-GC-APG (which remained normal) in OA rabbits. The normal scintigraphic ratios of 99mTc-J001 in sham-operated and non-operated rabbits, as well as of 99mTc-GC-APG in OA rabbits, suggested that the increased uptake demonstrated with 99mTc-J001 in OA rabbits, as early as day 18 corresponded to imaging of synovitis via elective macrophage targeting. These results showed that 99mTc-J001 scintigraphy should be a specific method of detecting synovitis in OA.
在15只患有右膝骨关节炎（OA，交叉韧带切断）的兔子、5只假手术兔子和4只未手术兔子中，研究了用99m锝标记的J001（99mTc-J001）闪烁扫描检测滑膜炎的潜力。J001是一种从肺炎克雷伯菌非致病菌株的膜中分离出的无热原性、酰化聚（1,3）半乳糖苷，它能够通过与CD11b和CD14分子结合而选择性地与巨噬细胞结合。将99mTc-J001闪烁扫描的结果与99m锝标记的亚甲基二膦酸盐（99mTc-MDP）闪烁扫描和GC-APG（J001的一种衍生物，在体外不能结合巨噬细胞）闪烁扫描的结果进行比较。早在第18天到第90天，OA兔子中99mTc-J001的平均闪烁扫描比率（患病膝关节与健康膝关节）显著高于假手术和未手术的兔子。99mTc-J001闪烁扫描显示摄取增加的时间比99mTc-MDP闪烁扫描更早。在OA兔子中，99mTc-J001的平均闪烁扫描比率显著高于99mTc-GC-APG（其保持正常）的平均闪烁扫描比率。假手术和未手术兔子中99mTc-J001的正常闪烁扫描比率，以及OA兔子中99mTc-GC-APG的正常闪烁扫描比率，表明早在第18天，OA兔子中99mTc-J001显示的摄取增加对应于通过选择性巨噬细胞靶向对滑膜炎的成像。这些结果表明，99mTc-J001闪烁扫描应该是检测OA中滑膜炎的一种特异性方法。
