携带串联插入汞抗性操纵子的重组质粒的大肠杆菌HB101和恶臭假单胞菌PpY101的特性。

Characteristics of Escherichia coli HB101 and Pseudomonas putida PpY101 harboring a recombinant plasmid with tandem insertion of the mercury resistance operon.

作者信息

Kurabayashi T, Iwasaki K, Uchiyama H, Nakamura K, Tanaka H, Yagi O

机构信息

National Institute for Environmental Studies, Ibaraki, Japan.

出版信息

Biosci Biotechnol Biochem. 1997 Jul;61(7):1187-9. doi: 10.1271/bbb.61.1187.

DOI:10.1271/bbb.61.1187

PMID:9255983

Abstract

We constructed the plasmid pSUPmer2 by inserting tandem copies of the mercury resistance (mer) operon into a broad host range-vector, and introduced it into Escherichia coli HB101 and Pseudomonas putida PpY101 to increase their mercury resistance. Strains harboring plasmid pSUPmer2 had higher mercury resistance and mercuric reductase activity than those strains harboring the plasmid pSUPmer which had one copy of the mer operon. Mercury resistance of P. putida PpY101 was significantly increased by tandem insertion of the mer operon.

摘要

我们通过将汞抗性（mer）操纵子的串联拷贝插入一个广宿主范围载体中构建了质粒pSUPmer2，并将其导入大肠杆菌HB101和恶臭假单胞菌PpY101以提高它们的汞抗性。携带质粒pSUPmer2的菌株比携带含有一个mer操纵子拷贝的质粒pSUPmer的菌株具有更高的汞抗性和汞还原酶活性。mer操纵子的串联插入显著提高了恶臭假单胞菌PpY101的汞抗性。