Niculescu D, Popa M, Stavri H, Teodor I, Stavri D, Dorobăţ O, Cozma G, Homoş M, Erşcoiu S
Cantacuzino Institute, Bucharest, Romania.
Roum Arch Microbiol Immunol. 1996 Jul-Sep;55(3):205-14.
IgG antibodies against glycolipids and proteins isolated from M. tuberculosis and BCG suspension were determined by ELISA in sera, in CSFs and in serum and CSF paired samples, from patients with tuberculous meningitis and from healthy control subjects. With specificities between 90 and 94% for the antigens used, we obtained senitivities of 75% for Pr-ELISA, 60% for G1-ELISA and 35% for BCG-ELISA. As specific antibodies were detected in serum and CSFs, only one sample is enough to perform the test. We concluded that Pr-ELISA and G1-ELISA could be used as a supporting test in TBM diagnosis, especially when repeated bacteriological methods failed to prove the presence of tubercle bacilli and in cases without evidence of pulmonary tuberculosis.
通过酶联免疫吸附测定（ELISA），在患有结核性脑膜炎的患者以及健康对照者的血清、脑脊液以及血清和脑脊液配对样本中，测定了针对从结核分枝杆菌和卡介苗悬液中分离出的糖脂和蛋白质的IgG抗体。对于所使用的抗原，特异性在90%至94%之间，我们获得的Pr-ELISA敏感性为75%，G1-ELISA为60%，卡介苗ELISA为35%。由于在血清和脑脊液中检测到了特异性抗体，因此只需一个样本就足以进行该检测。我们得出结论，Pr-ELISA和G1-ELISA可作为结核性脑膜炎诊断的辅助检测方法，特别是在反复的细菌学方法未能证明结核杆菌存在以及无肺结核证据的情况下。
