Fellier H, Gleispach H, Esterbauer H
J Clin Chem Clin Biochem. 1977 Oct;15(10):545-8.
The binding properties of [3H]cortisol, [3H]fluocortolone and [3H]difluocortolone by human plasma, human albumin, human- beta- and gamma-globulins have been studied by equilibrium dialysis. Cortisol, in physiological concentrations (0,4 micromol/l), is 98% bound in human plasma at 25 degrees C, fluocortolone 96% and diflucortolone 85%. Uncer physiological conditions cortisol is mainly bound to the corticosteroid binding globulin (transcortin). 2/3 of fluocortolone is bound to transcortin and 1/3 to albumin and globulins, whereas difluocortolone is mainly bound to albumin and to globulins but not to transcortin. The binding affinities of beta- and gamma-globulins are -ery low for the corticoids investigated, but they are higher for fluocortolone and difluocortolone than for cortisol.
通过平衡透析研究了[3H]皮质醇、[3H]氟氢可的松和[3H]二氟氢可的松与人血浆、人白蛋白、人β球蛋白和γ球蛋白的结合特性。在25℃时，生理浓度（0.4微摩尔/升）的皮质醇在人血浆中的结合率为98%，氟氢可的松为96%，二氟氢可的松为85%。在生理条件下，皮质醇主要与皮质类固醇结合球蛋白（皮质素转运蛋白）结合。氟氢可的松的2/3与皮质素转运蛋白结合，1/3与白蛋白和球蛋白结合，而二氟氢可的松主要与白蛋白和球蛋白结合，但不与皮质素转运蛋白结合。所研究的皮质类固醇与β球蛋白和γ球蛋白的结合亲和力非常低，但氟氢可的松和二氟氢可的松的结合亲和力高于皮质醇。
