Schwartz J L, Lu Y J, Söhnlein P, Brousseau R, Laprade R, Masson L, Adang M J
Biotechnology Research Institute, National Research Council, Montreal, Que., Canada.
FEBS Lett. 1997 Jul 28;412(2):270-6. doi: 10.1016/s0014-5793(97)00801-6.
A purified, GPI-linked receptor complex isolated from Manduca sexta midgut epithelial cells was reconstituted in planar lipid bilayers. CryIAa, CryIAc and CryIC, three Bacillus thuringiensis insecticidal proteins, formed channels at much lower doses (0.33-1.7 nM) than in receptor-free membranes. The non-toxic protein CryIB also formed channels, but at doses exceeding 80 nM. The channels of CrylAc, the most potent toxin against M. sexta, rectified the passage of cations. All other toxin channels displayed linear current-voltage relationships. Therefore, reconstituted Cry receptors catalyzed channel formation in phospholipid membranes and, in two cases, were involved in altering their biophysical properties.
从烟草天蛾中肠上皮细胞分离出的一种纯化的、糖基磷脂酰肌醇连接的受体复合物，被重组到平面脂质双分子层中。苏云金芽孢杆菌的三种杀虫蛋白CryIAa、CryIAc和CryIC，在比无受体膜低得多的剂量（0.33 - 1.7 nM）下形成通道。无毒蛋白CryIB也形成通道，但剂量超过80 nM。对烟草天蛾毒性最强的毒素CrylAc的通道使阳离子的通过发生整流。所有其他毒素通道呈现线性电流-电压关系。因此，重组的Cry受体催化了磷脂膜中通道的形成，并且在两种情况下，参与改变了它们的生物物理特性。
