CD95配体对同种抗体反应的抑制作用。

Inhibition of the alloantibody response by CD95 ligand.

作者信息

Arai H, Chan S Y, Bishop D K, Nabel G J

机构信息

Howard Hughes Medical Institute, University of Michigan Medical Center, Department of Internal Medicine, Ann Arbor 48109-0650, USA.

出版信息

Nat Med. 1997 Aug;3(8):843-8. doi: 10.1038/nm0897-843.

DOI:10.1038/nm0897-843

PMID:9256273

Abstract

We investigated the effect of Fas/APO1-ligand (CD95L) gene transfer on allogeneic immune responses in vivo. A colon carcinoma cell line from BALB/c mice, CT26, was stably transfected with a vector encoding mouse CD95L and was inoculated into C57BL/6 mice. CD95L expression markedly reduced allogeneic cytotoxic T lymphocyte and helper T lymphocyte activity directed toward CT26. Strikingly, expression of CD95L on these allogeneic tumors completely inhibited the generation of alloantibodies of both IgM and IgG subclasses. Thus, CD95L inhibited alloantibody production and conferred localized immune suppression through this mechanism. These results provide insight into the role of CD95L in regulating the alloantibody response and the generation of local immune responses.

摘要

我们研究了Fas/APO1配体（CD95L）基因转移对体内同种异体免疫反应的影响。将编码小鼠CD95L的载体稳定转染至来自BALB/c小鼠的结肠癌细胞系CT26，并将其接种到C57BL/6小鼠体内。CD95L的表达显著降低了针对CT26的同种异体细胞毒性T淋巴细胞和辅助性T淋巴细胞活性。令人惊讶的是，这些同种异体肿瘤上CD95L的表达完全抑制了IgM和IgG亚类同种抗体的产生。因此，CD95L通过该机制抑制同种抗体产生并赋予局部免疫抑制作用。这些结果为CD95L在调节同种抗体反应和局部免疫反应产生中的作用提供了深入了解。