Maezawa H, Komatsu H, Kawaoi A, Ueno A
Department of Urology, Yamanashi Medical University, Nakakoma-gun, Japan.
Int J Urol. 1997 Jul;4(4):411-6. doi: 10.1111/j.1442-2042.1997.tb00217.x.
We used buserelin acetate ([D-Ser(But)6] LHRH ), an LHRH agonist and strong blocker of LH secretion, as a treatment for prostatic cancer. It is possible that this LHRH agonist has a proliferative effect on the prostate in addition to suppressing LH secretion. The purpose of this study was to examine the proliferative effect of LHRH agonist on rat prostatic epithelial cells.
We determined the optimal dose of testosterone necessary to maintain a positive level of proliferating cell nuclear antigen (PCNA) in the ventral prostatic epithelial cells of castrated Wistar rats. Testosterone-treated rats then received various doses of buserelin acetate. Castrated rats without exogenous testosterone also received buserelin acetate. The PCNA positivity was determined by immunohistochemistry with anti-PCNA monoclonal antibody.
The optimal dose of testosterone enanthate was 4 mg at 0 and 28 days after castration. Administration of buserelin acetate on day 0 and 28 in doses of 0.16 mg to 1.28 mg significantly increased PCNA positivity in a dose-dependent manner. Administration of buserelin acetate to castrated rats without testosterone also increased PCNA positivity but there was no statistical significance.
Buserelin acetate has a potentiating effect on the proliferation of ventral prostatic epithelial cells of castrated rat in the presence of a physiological level of exogenous testosterone. This effect may slightly influence the result of hormonal therapy by LHRH agonist.
我们使用醋酸布舍瑞林（[D-丝氨酸（叔丁基）6]促黄体激素释放激素），一种促黄体激素释放激素激动剂和促黄体生成素分泌的强效阻滞剂，作为前列腺癌的治疗药物。除抑制促黄体生成素分泌外，这种促黄体激素释放激素激动剂可能对前列腺有增殖作用。本研究的目的是检测促黄体激素释放激素激动剂对大鼠前列腺上皮细胞的增殖作用。
我们确定了维持去势Wistar大鼠腹侧前列腺上皮细胞增殖细胞核抗原（PCNA）阳性水平所需的睾酮最佳剂量。然后，用睾酮处理的大鼠接受不同剂量的醋酸布舍瑞林。未接受外源性睾酮的去势大鼠也接受醋酸布舍瑞林。通过抗PCNA单克隆抗体免疫组织化学法测定PCNA阳性率。
在去势后0天和28天，庚酸睾酮的最佳剂量为4mg。在第0天和第28天给予0.16mg至1.28mg剂量的醋酸布舍瑞林可显著增加PCNA阳性率，且呈剂量依赖性。对未接受睾酮的去势大鼠给予醋酸布舍瑞林也可增加PCNA阳性率，但无统计学意义。
在存在生理水平外源性睾酮的情况下，醋酸布舍瑞林对去势大鼠腹侧前列腺上皮细胞的增殖有增强作用。这种作用可能会对促黄体激素释放激素激动剂的激素治疗结果产生轻微影响。
