Freise J, Magerstedt P, Schmidt E
J Clin Chem Clin Biochem. 1977 Sep;15(9):485-8.
The method for the determination of enzymic activity in turbid, lipaemic sera, which involves clearing by polyanion precipitation with heparin and magnesium chloride, was critically reviewed. In the diagnosis of diseases of the liver and pancreas, which are frequently associated with hyperlipoproteinaemia, only residual enzyme activities are measured in the cleared serum after polyanion treatment. In the measurement of glutamate dehydrogenase and in the Phadebas test for alpha-amylase, the enzymes are inactivated by treatment with heparin and magnesium chloride. On the other hand, as a result of polyanion precipitation gamma-glutamyl transferase is transferred, together with lipoproteins and chylomicrons, to the lipid-rich supernatant. Acid phosphatase also exhibits only residual activity in cleared serum. The activity of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, leucine arylamidase, cholinesterase, creatine kinase, lactate dehydrogenase, and alpha-hydroxybutyrate dehydrogenase, and the activity of alpha-amylase in the Merckotest are not affected by polyanion treatment of the serum.
对通过用肝素和氯化镁进行聚阴离子沉淀来清除浑浊、脂血血清中酶活性的方法进行了严格审查。在肝脏和胰腺疾病的诊断中，这些疾病常与高脂蛋白血症相关，聚阴离子处理后，仅测定清除血清中的残余酶活性。在谷氨酸脱氢酶的测定以及α-淀粉酶的Phadebas试验中，酶会因肝素和氯化镁处理而失活。另一方面，由于聚阴离子沉淀，γ-谷氨酰转移酶与脂蛋白和乳糜微粒一起转移到富含脂质的上清液中。酸性磷酸酶在清除血清中也仅表现出残余活性。血清经聚阴离子处理后，天冬氨酸氨基转移酶、丙氨酸氨基转移酶、碱性磷酸酶、亮氨酸芳基酰胺酶、胆碱酯酶、肌酸激酶、乳酸脱氢酶和α-羟丁酸脱氢酶的活性，以及默克试验中α-淀粉酶的活性不受影响。
